BIOL-211 Molecular Biotechnology

Credits 4 / 6 Contact Hours
Prerequisite:Pre-requisite: Placement into RDNG-030 (ACSR-030) or successful completion of RDNG-016 or ACLT-074 with a
minimum grade of 3.0. Prerequisite: BIOL-111
A lecture and lab combination course utilizing state of the art techniques in molecular biology and recombinant DNA technology in the investigation of general concepts in genetics and the structure and function of DNA as a hereditary molecule. The students will be oriented to the use of laboratory equipment and procedures that are currently used in biotechnology.

Course Outcomes
1. Demonstrate knowledge of the structure of DNA 2. Build a model of DNA 3. Demonstrate proficiency at macro- and micro-pipetting fluids 4. Prepare bacteriological media 5. Prepare and maintain bacterial cultures 6. Demonstrate proficiency at preparation of agarose gel 7. Electrophorese and separate DNA molecules of various sizes 8. Demonstrate knowledge of the activity and specificity of several restriction endonucleases 9. Carry out restriction digests of DNA samples 10. Isolate plasmid DNA from bacterial cells 11. Transform bacterial cells to antibiotic resistance with plasmid DNA 12. Construct recombinant plasmid 13. Isolate high molecular weight DNA from animal tissues 14. Detect specific DNA sequences on a Southern blot utilizing non-radioactive nucleic acid probes